Table 3.

Experimental parameters for Capflex analysis of α-Syn on the FIDA 1 instrument.

Tray	Vial	Pressure (mbar)	Time (s)	Outlet	Measure	Comment
2	1	3500	45	Variable	No	1 M NaOH wash
2	2	3500	60	Variable	No	milliQ wash
1	Indicator	3500	40	Variable	No	Buffer wash
1	Analyte	1200 or 2000	600	Variable	Yes	Sample application

Trays 1 and 2 were maintained at 37 °C and the capillary chamber was also maintained at 37 °C. Since this system consisted of 20% PEG6000, due to high viscosity, the sample application was performed with a relatively higher pressure (1200 or 2000 mbar).