Table 5.
Method for FIDA analyses for affinity determination of Ddx4n1 and ssDNA.
| Tray | Vial | Pressure (mbar) | Time (s) | Outlet | Measure | Comment |
|---|---|---|---|---|---|---|
| 2 | 1 | 3500 | 45 | Variable | No | 1 M NaOH wash |
| 2 | 2 | 3500 | 60 | Variable | No | milliQ wash |
| 1 | 48 | 3500 | 60 | Variable | No | 1% Tween coat |
| 1 | 46 | 3500 | 40 | Variable | No | Buffer wash |
| 1 | Analyte | 3500 | 20 | Variable | No | Analyte capillary fill |
| 1 | Indicator | 50 | 10 | Variable | No | Indicator-plug inject |
| 1 | Analyte | 400 | 180 | Variable | Yes | Mobilize and measure |
Tray 1 and 2 was maintained at 20 °C and the capillary chamber was also maintained at 20 °C for all our measurements.