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. 2021 Dec 15;12:7312. doi: 10.1038/s41467-021-27616-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Cumulative number of non-synonymous mutations in rpoC over time. The x-axis represents the years since the inferred time of rifampcin resistance (time 0). Dark blue line shows the cumulative number of mutations for the ML tree, while the 95% confidence interval (shaded area) is inferred by repeating the analysis in 100 bootstrap phylogenies. The analysis was performed separately for a lineage 2 and b lineage 4.