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. 2021 Dec 15;12:7305. doi: 10.1038/s41467-021-27542-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — The left panel displays the results for the SIHUMIx sample S11 (A), while the right panel corresponds to the results for the fecal sample F07 (B). The four different bioinformatic pipelines (MetaProteomeAnalyzer (MPA, using X!Tandem and OMSSA), Proteome Discoverer (PD, using SequestHT), MaxQuant (MQ, using Andromeda), SearchGUI/PeptideShaker (PS, using X!Tandem, OMSSA, MS-GF+, and Comet)) are indicated on the x-axis and sorted by increasing set size. Set size corresponds to the total number of peptides identified per tool, and intersection size corresponds to the number of shared peptides identified in different approaches. Green highlights the intersection, and blue shows unique peptides to each tool. The lower panel box plots show peptide lengths, and number of missed cleavages for each intersection. Source data is provided as a Source Data file.