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. 2021 Dec 15;12:7293. doi: 10.1038/s41467-021-27488-x

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021

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Fig. 7 — a–c Changes in levels of Nile red (a), BODIPY (b), and APOE (c) stained sub-RPE deposits in CFH(Y/Y), CFH(Y/H), CFH(H/H) cotreated with CC-HS plus L745 or ACA, compared to CC-HS plus vehicle cotreated cells (N = 6 biologically independent replicates, iRPE1, 4, 5, 6, 7, 8). P values were determined by multiple pairwise comparisons using two-tailed t test with 95% confidence interval and Bonferroni correction. The horizontal lines in the boxplots indicate the median, the boxes indicate the first and third quartiles, and the whiskers indicate 5th and 95th percentile. d APOE-positive sub-RPE deposits in CFH(Y/Y), CFH(Y/H), CFH(H/H) treated with vehicle, L745 or ACA for 7 days after 24 h CC-HS treatment, compared with a 48-h CC-HS + vehicle treatment (N = 6 biologically independent replicates, iRPE1, 4, 5, 6, 7, 8). P values were determined by multiple pairwise comparisons using two-tailed t test with 95% confidence interval and Bonferroni correction. The horizontal lines in the boxplots indicate the median, the boxes indicate the first and third quartiles, and the whiskers indicate 5th and 95th percentile. e–l Immunostaining for VIMENTIN (red) shows localization defect induced by CC-HS treatment is restored by cotreatment of CC-HS with L745 or ACA. F-ACTIN (green) stains the cytoskeleton (N = 5 biologically independent replicates, iRPE1, 3, 5, 6, 7). m, n Cotreatment of CC-HS with drugs L745 or ACA rescued CC-HS induced TER drop (m) and improved phagocytic ability (n) in CFH(Y/Y) and CFH(H/H)-iRPE (N = 6 biologically independent replicates, iRPE1, 2, 3, 5, 6, 7). P values were determined by multiple pairwise comparisons using two-tailed t test with 95% confidence interval and Bonferroni correction. The horizontal lines in the boxplots indicate the median, the boxes indicate the first and third quartiles, and the whiskers indicate 5th and 95th percentile. o, p Nile red (o) and BODIPY (p) positive sub-RPE lipid deposits in CFH(Y/Y) and CFH(H/H)-iRPE treated with vehicle, L745 or ACA for 24 h after a 24-h CC-HS treatment (N = 6 biologically independent replicates, 1, 4, 5, 6, 7, 8). P values were determined by multiple pairwise comparisons using two-tailed t test with 95% confidence interval and Bonferroni correction. The horizontal lines in the boxplots indicate the median, the boxes indicate the first and third quartiles, and the whiskers indicate 5th and 95th percentile.