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. 2021 Dec 15;12:7310. doi: 10.1038/s41467-021-27597-7

Fig. 4. Phospho-oligomeric state governs IRE1α‘s RNase modality.

Fig. 4

a DSS crosslinking of IRE1-KR-0P and IRE1-KR-3P at various concentrations. (All lanes have the same final amount of protein loaded.) b Cartoon depicting the procedure to extract KR-3P fractions before incubation with T7 RNAs. c Digestion of XBP1u, DGAT2, and TNFAIP8L1 RNA by isolated fractions of IRE1-KR-3P: M, monomer; D, dimer; and O, oligomer. d WT structure of IRE1α dimer with the residues of interest highlighted: alignment of yeast B2B IRE1α (PDB ID: 3FBV, green cartoon) and human B2B IRE1α (PDB ID: 6W3C, magenta cartoon). e Alignment of yeast oligomeric IRE1α (PDB ID: 3FBV, green cartoon) and two dimers of human IRE1α (PDB ID: 6W3C, magenta and blue cartoons). Inset: R887-E876 spatial arrangement based on the alignment. f DSS crosslinking of purified recombinant IRE1-KR-3P WT and R887A. g Digestion of DGAT2 and TNFAIP8L1 RNA by IRE1-KR-3P WT and R887A.