Table 2.
CircRNA biomarkers in blood cells or whole blood.
| Disease | CircRNA biomarker | Source | Expression change | Cohort size | Clinical significance | AUC | Method | Reference |
|---|---|---|---|---|---|---|---|---|
| AIS | Circ-DLGAP4 | PBMC | Down | 170 AIS /170 HC | Associated with Health Stroke Scale score and levels of C-reactive protein, TNF-α, IL-6, IL-8, IL-22. | 0.816 | RT-qPCR | Zhu et al. (2019b) |
| ALS |
Hsa_circ_0023919 Hsa_circ_0063411 Hsa_circ_0088036 |
Leukocyte |
Down Up Up |
60 ALS/15 HC | Hsa_circ_0063411 was associated with the disease duration and survival time. | 0.950 |
Microarray RT-qPCR |
Dolinar et al. (2019) |
| AML | Hsa_circ_0004277 | Mononuclear cell | Down | 115 AML/12 HC | Associated with progressive stage. | 0.957 |
Microarray RT-qPCR |
Li et al. (2017c) |
| CAD | CircZNF609 | Leukocyte | Down | 330 CAD/209 HC | Associated with inflammatory processes. | 0.761 | RT-qPCR | Liang et al. (2020) |
| CAD | Hsa_circ_0001879 Hsa_circ_0004104 | PBM | Up | 436 CAD/297 HC | Hsa_circ_0001879 was associated with body mass index (BMI), systolic blood pressure, diastolic blood pressure and Gensini score; hsa_circ_0004104 was associated with high-density lipoprotein cholesterol. |
0.703 0.700 |
Microarray RT-qPCR |
Wang et al. (2019a) |
| CAD | Hsa_circ_0124644 | Whole blood | Up | 179 CAD/157 HC | Associated with severity. | 0.769 |
Microarray RT-qPCR |
Zhao et al. (2017a) |
| CAP |
Hsa_circ_0018429 Hsa_circ_0026579 Hsa_circ_0099188 Hsa_circ_0012535 |
Whole blood | Up | 36 CAP/36 HC | NA | 0.878 |
Microarray RT-qPCR |
Zhao et al. (2019) |
| CML | Hsa_circ_100053 | PBMC | Up | 150 CML/100 HC | Associated with clinical stage, BCR/ABL mutant status, imatinib response and prognosis. | NA |
RNA-seq RT-qPCR |
Ping et al. (2019) |
| CSCC |
Hsa_circ_0101996 Hsa_circ_0101119 |
Whole blood | Up | 87 CSCC/55 HC | NA | 0.964 | RT-qPCR | Wang et al. (2017a) |
| EH | Hsa_circ_0037911 | Whole blood | Up | 100 EH/100 HC | Associated with gender, smoking, drinking and serum creatinine. | 0.627 |
Microarray RT-qPCR |
Bao et al. (2018) |
| EH | Hsa_circ_0037909 | Whole blood | Up | 48 EH/48 HC | Associated with serum creatinine and low‐density lipoprotein. | 0.682 | RT-qPCR | Bao et al. (2019) |
| EH | Hsa_circ_0014243 | Whole blood | Up | 89 EH/89 HC | Associated with age, high-density lipoprotein level and glucose level. | 0.732 | RT-qPCR | Zheng et al. (2019) |
| EH | Hsa_circ_91025 | Whole blood | Up | 96 EH/96 HC | Associated with high‐density lipoprotein, BMI, diastolic blood pressure and systolic blood pressure. | 0.620 | RT-qPCR | Zheng et al. (2020) |
| GC | Hsa_circ_0001821 | Whole blood | Down | 30 GC/30 HC | NA | 0.872 | RT-qPCR | Kong et al. (2019) |
| HCC | Hsa_circ_0000798 | PBMC | Up | 72 HCC/30 HC | Associated with tumor size, cirrhosis and overall survival. | 0.703 |
RNA-seq RT-qPCR |
Lei et al. (2019) |
| HT | Hsa_circ_0089172 | PBMC | Up | 35 HT/35 HC | Associated with the serum level of the thyroid peroxidase antibody. | 0.715 |
RNA-seq RT-qPCR |
Xiong et al. (2019) |
| IA | Hsa_circ_0021001 | Whole blood | Down | 223 IA/131 HC | Associated with aneurysm rupture, Hunt, Hess level, timing of surgery, disease-free survival and overall survival. | 0.870 | RT-qPCR | Teng et al. (2017) |
| MI | MICRA | Whole blood | Down | 642 Acute MI/86 HC | Associated with the risk of left ventricular dysfunction. | NA |
Microarray RT-qPCR |
Vausort et al. (2016) |
| MS | Hsa_circ_0005402 Hsa_circ_0035560 | Leucocyte | Down | 45 MS/26 HC | NA |
0.899 0.706 |
Microarray RT-qPCR |
Iparraguirre et al. (2017) |
| NSCLC | Hsa_circ_0102533 | Whole blood | Up | 41 NSCLC/26 HC | Associated with tumor type, TNM stage, LNM and distant metastasis. | 0.774 |
Microarray RT-qPCR |
Zhou et al. (2018b) |
| OA | Hsa_circ_0032131 | Whole blood | Up | 25 OA/25 HC | Associated with the pathological process. | 0.846 |
Microarray RT-qPCR |
Wang et al. (2019f) |
|
KBD / OA |
Hsa_circ_0020014 | Whole blood | Down | 25 KBD/25 OA | Associated with early diagnosis of OA and KBD. | 0.642 |
Microarray RT-qPCR |
Wang et al. (2020e) |
| PE | Hsa_circ_0004904 Hsa_circ_0001855 | Whole blood | Up | 35 PE/35 HC | Associated with serum pregnancy-associated plasma protein A level. |
0.611 0.621 |
Microarray RT-qPCR |
Jiang et al. (2018) |
| PE | Hsa_circ_101222 | Blood corpuscle | Up | 41 PE/41 HC | NA | 0.706 |
Microarray RT-qPCR |
Zhang et al. (2016) |
| PMOP | Hsa_circ_0001275 | PBMC | Up | 58 PMOP/41 HC | Associated with T-score. | 0.759 |
Microarray RT-qPCR |
Zhao et al. (2018a) |
| RA | Hsa_circ_0044235 | Whole blood | Down | 77 RA/31 SLE/50 HC | NA | 0.779 | RT-qPCR | Luo et al. (2018) |
| RA |
Hsa_circ_104871 Hsa_circ_003524 Hsa_circ_101873 Hsa_circ_103047 |
PBMC | Up | 35 RA/30 HC | NA |
0.833 0.683 0.676 0.671 |
Microarray RT-qPCR |
Ouyang et al. (2017) |
| RA |
Hsa_circ_0000396 Hsa_circ_0130438 |
PBMC | Down | 32 RA/20 HC | NA |
0.809 0.774 |
RNA-seq RT-qPCR |
Yang et al. (2019) |
| SLE | Hsa_circ_0000479 | PBMC | Up | 97 SLE/50 RA/89 HC | Associated with albumin level, urine protein, IgG, leukocytes, hemoglobin and ESR. | 0.731 |
RNA-seq RT-qPCR |
Guo et al. (2019) |
| SLE |
Hsa_circ_0057762 Hsa_circ_0003090 |
Whole blood | Up | 24 SLE/24 HC | Hsa_circ_0057762 was associated with the SLEDAI-2K score. |
0.804 0.848 |
Microarray RT-qPCR |
Li et al. (2019b) |
| SLE | Hsa_circ_0044235 Hsa_circ_0068367 | PBMC | Down | 79 SLE/30 RA/62 HC | Hsa_circ_0044235 was associated with the numbers of monocytes and autoantibodies. |
0.873 0.768 |
Microarray RT-qPCR |
Luo et al. (2019) |
| SLE | CircPTPN22 | PBMC | Down | 53 SLE/40 HC | Associated with SLE activity index scores. | 0.918 |
RNA-seq RT-qPCR |
Miao et al. (2019) |
| SLE |
Hsa_circ_407176 Hsa_circ_001308 |
PBMC | Down | 126 SLE/102 HC | NA |
0.806 0.722 |
Microarray RT-qPCR |
Zhang et al. (2018e) |
| SLE | Hsa_circ_0012919 | CD4+ T cell | Up | 28 SLE/18 HC | Associated with clinical and lab features. | – |
Microarray RT-qPCR |
Zhang et al. (2018a) |
| Schizophrenia | Hsa_circ_104597 | PBMC | Down | 102 Schizophrenia/103 HC | Significant expression difference between pre-treatment and post-treatment. | 0.885 |
Microarray RT-qPCR |
Yao et al. (2019) |
| T2DM | CircANKRD36 | Leucocyte | Up | 43 T2DM/45 HC | Associated with glucose, glycosylated hemoglobin and interleukin. | NA |
RNA-seq RT-qPCR |
Fang et al. (2018) |
| T2DM | Hsa_circ_0054633 | Whole blood | Up | 90 T2DM/83 Pre-diabetes/86 HC | NA | 0.751 |
Microarray RT-qPCR |
Zhao et al. (2017b) |
| TB |
Hsa_circ_103017 Hsa_circ_059914 Hsa_circ_101128 |
PBMC | Up | 31 TB/30 HC | Hsa_circ_101128 was associated with the level of let‐7a. |
0.870 0.821 0.817 |
Microarray RT-qPCR |
Fu et al. (2019) |
| TB |
Hsa_circ_0043497 Hsa_circ_0001204 |
Monocyte | Up | 101 TB/88 HC | Significant expression difference between pre-treatment and post-treatment. |
0.860 0.848 |
Microarray RT-qPCR |
Huang et al., (2017c) |
| TB | Hsa_circ_001937 | PBMC | Up | 178 TB/40 PE/40 COPD/40 LC/133 HC | Associated with radiological scores; significant expression difference between pre-treatment and post-treatment. | 0.873 |
Microarray RT-qPCR |
Huang et al., (2018c) |
| TB | Hsa_circ_0001380 | PBMC | Down | 32 TB/31 HC | NA | 0.950 | RT-qPCR | Luo et al. (2020b) |
| TB |
Hsa_circ_0000414 Hsa_circ_0000681 Hsa_circ_0002113 Hsa_circ_0002362 Hsa_circ_0002908 Hsa_circ_0008797 Hsa_circ_0063179 |
PBMC | Up | 12 TB/13 HC | NA | 0.946 |
RNA-seq Microarray RT-qPCR |
Qian et al. (2018) |
| TB | Hsa_circ_0005836 | PBMC | Down | 49 TB/45 HC | NA | NA |
RNA-seq RT-qPCR |
(Zhuang et al., 2017) |
Abbreviation: AIS: acute ischemic stroke; ALS: amyotrophic lateral sclerosis; AML: acute myeloid leukemia; CAD: coronary artery disease; CAP: community‐acquired pneumonia; CML: chronic myeloid leukemia; COPD: chronic obstructive pulmonary disease; CSCC: cervical squamous cell carcinoma; EH: essential hypertension; GC: gastric cancer; HC: healthy control; HCC: hepatocellular carcinoma; HT: Hashimoto’s thyroiditis; IA: intracranial aneurysm; KBD: Kashin-Beck disease; MI: myocardial infarction; MS: multiple sclerosis; NA: not applicable; NSCLC: non-small cell lung cancer; OA: osteoarthritis; PBMC: peripheral blood mononuclear cell; PE: pre-eclampsia; PMOP: postmenopausal osteoporosis; RA: rheumatoid arthritis; SLE: systemic lupus erythematosus; T2DM: type 2 diabetes mellitus; TB: tuberculosis.