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. 2021 Dec 15;11:168. doi: 10.1186/s13568-021-01330-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Effect of SpeA, SpeB, and SpeC on putrescine production. A Bacterial cell growth and B putrescine concentration in the supernatant of strains FS123 (speC⁺, open circle), KN20 (speABC⁺, closed triangle), and KN24 (speAB⁺, closed square) grown in LB medium supplemented with 100 μg/mL of ampicillin. When the OD₆₀₀ reached 0.4, 0.02 mM IPTG was added. Data shown are averages ± standard deviations, and culture experiments were performed in triplicate