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. 2021 Dec 15;11:168. doi: 10.1186/s13568-021-01330-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Effects of patA, puuA, and puuP genes on the putrescine production. The amount of putrescine in the culture supernatant of the strains KT148, KT152, KT153, KT154, and KT162 grown in LB medium supplemented with 100 μg/mL of ampicillin for 48 h. When the OD₆₀₀ reached 0.4, 0.03 mM IPTG was added. ***, p < 0.001; ns, not significantly different. Data shown are averages ± standard deviations, and culture experiments were performed in triplicate