Table 2.
Plasmids used in this study
| Strains | Genotype | Source or reference |
|---|---|---|
| pCP20 | pSC101 replicon (Ts) bla+ cat+ | Datsenko and Wanner 2000 |
| Flp(γRp) d857 | ||
| pFS29 | pQE-80L except T5p lacO_lacO_speC+ | This study |
| pKD13 | oriRγ bla+ FRT-kanR-FRT | Datsenko and Wanner 2000 |
| pKN11 | pQE-80L except T5p lacO_lacO | This study |
| _speA_speB_argAATG Y19C | ||
| pKN18 | pQE-80L except T5p lacO_lacO | This study |
| _speA_speB_argAATG Y19C T5p lacO_ | ||
| lacO_speC | ||
| pKT199 | pQE-80L except | This study |
| lacIq1p_lacO_speA_speB_argAGTG Y19C | ||
| lacI | ||
| pKT200 | pQE-80L except | This study |
| lacIq1p_lacO_speA_speB_argAATG Y19 | ||
| lacI | ||
| pKT201 | pQE-80L except | This study |
| lacIq1p_lacO_speA_speB_argAGTG Y19 | ||
| lacI | ||
| pQE-80L | ColE1 replicon bla+ lacIq T5p | Qiagen |
| lacO_lacO-(His)6 | ||
| pSH1733 | pQE-80L except argAATG Y19C | This study |
| pSH1734 | pQE-80L except T5p lacO_lacO_speC+ | This study |
| _ argAATG Y19C | ||
| pSH1807 | pQE-80L except | This study |
| lacIqp_lacO_speA_speB_argAATG Y19C | ||
| lacI | ||
| pSH1820 | pQE-80L except | This study |
| lacIq1p_lacO_speA_speB_argAATG Y19C | ||
| lacI | ||
| pSH1823 | pQE-80L except | This study |
| lacIp_lacO_speA_speB_argAATG Y19C | ||
| lacI |