FIG. 1.

MseI restriction patterns of B. burgdorferi sensu lato rrf-rrl spacer nested PCR products amplified directly from ticks or cultured strains. Lanes 1 and 10, D-15 marker; lanes 2 and 3, cultured B. burgdorferi sensu stricto (ZS 7) and B. lusitaniae (PotiB1), patterns A and E, respectively; lanes 4 and 5, tick-derived samples 58 and 151 (pattern E), respectively; Lane 6, tick-derived sample 132 (pattern F); lane 7, tick-derived sample 163 (patterns E and F); lane 8, tick-derived sample 156 (pattern H); lane 9, tick-derived sample 167 (pattern G).