TABLE 2.
MseI restriction pattern of the rrf-rrl spacer of B. burgdorferi sensu lato amplified by nested PCR directly from ticks or cultured strains
| Strain or tick-derived samplea | Amplicon size (bp) | RFLP pattern | MseI restriction fragment size (bp)b |
|---|---|---|---|
| B. burgdorferi ZS7 | 227 | A | 95, 37, 29, 28 |
| B. afzelii ACA 1 | 220 | B | 20, 37, 68, 95 |
| B. garinii ZQ 1 | 227 | C | 95, 95, 37 |
| B. valaisiana UK | 229 | D | 162, 37, 23, 7 |
| B. lusitaniae PotiB1 | 230 | E | 95, 67, 39, 29 |
| GT058c (I)d | 230 | E | 95, 67, 39, 29 |
| GT078 (V) | 230 | E | 96, 66, 39, 29 |
| GT151 (VI) | 231 | E | 96, 67, 39, 29 |
| GT172 (VII) | 230 | E | 95, 67, 39, 29 |
| GT132 (IV) | 229 | F | 95, 66, 52, 16 |
| GT098 (II) | 212 | G | 95, 78, 39 |
| GT167 (VIII) | 212 | G | 95, 78, 39 |
| GT156 (III) | 220 | H | 95, 66, 30, 29 |
| GT158 (IX) | 213 | G+E | 96, 78, 39 |
| GT163 (X) | 230 | F+E | 95, 67, 29, 39 |
a
Only representative samples are shown.
b
The exact sizes of the fragments were determined from the sequence of the nested PCR product.
c
Sequence identical to that of PotiB1.
d
Roman numerals in parentheses indicate sequence type.