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. 2021 Dec 2;12:773013. doi: 10.3389/fimmu.2021.773013

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Copyright © 2021 Liu, Su and Chen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytotoxicity of TP4 in GV sup-induced THP-1 macrophages (THP-1/GV) and vaginal epithelial VK2 cells. THP-1/GV (A) and VK2 cells (B) were treated with different doses (0.49, 0.98, 1.95, 3.91, 7.82, 15.63 μg/ml) of TP4 for 24 h and subjected to MTS/PMS assay. Triton-X 100 (0.1%) served as a positive control (P). Results are shown as relative percentage to 0 μg/ml group. THP-1/GV (C) and VK2 (D) cells were treated with different doses (0.49, 0.98, 1.95, 3.91, 7.82, 15.63 μg/ml) of TP4 for 24 h. After treatment, the supernatants were subjected to an LDH release assay. Triton-X 100 (0.1%) served as a positive lysis control (P). Results are shown as relative percentage to positive control. Data are presented as mean ± SD of three independent experiments (**P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not statistically significant, compared to 0 μg/ml).