Figure 3.
Tryptase inhibition alters the mode of cell death in human mast cell leukemia cells subjected to cytotoxic agent. HMC-1 cells (0.5 x 106 cells/ml) were incubated with either Pefabloc SC (A; 100 μM) or nafamostat (B; 20 μM) for 30 min prior to treating the cells with LLME (400 μM) for 24 h. Cells were analyzed by flow cytometry for Annexin V/Draq7 positivity. Dot plots are shown with representative samples showing staining with Annexin V-FITC (FL-1) and Draq7 (FL-3), with the % of cells indicated within each quadrant. Quantification of the data is shown to the left. Data are representative of at least two independent experiments and are given as mean values ± SEM (n = 3). ***p ≤ 0.001.