Figure 6.
Tryptase is found in the nucleus of HMC-1 cells. HMC-1 cells (0.5 x 106 cell/ml) were either left untreated or treated with 400 μM LLME for 2 or 6 hours. Cells were stained for tryptase and nuclear DNA (Hoechst 33342), followed by confocal microscopy analysis. (A) 3-D view generated from Z-stack sections. Note the abundant tryptase staining in the cytoplasm of control cells and a gradual decline in tryptase staining after LLME treatment; bar scale = 10 μm. (B) The left and right panels show solid front and back views of the same control cell or cells treated with LLME for either 2 or 6 hours. The middle panels show translucent nuclear structure. Note that the translucent depiction of nuclei reveals the presence of tryptase within the nuclear compartment of both control- and LLME-treated cells (indicated by white arrows); bar scale = 5 μm.