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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Drug Discov Today Technol. 2021 Jul 9;39:49–56. doi: 10.1016/j.ddtec.2021.06.006

Figure 1.

Figure 1.

Schematic diagrams of SRM/MRM, PRM, DDA, and DIA proteomics. The SRM/MRM method isolates and fragments pre-defined precursor ions and monitors pre-defined product ions derived from the precursor. PRM isolates and fragments pre-defined precursors in the same way as SRM/MRM but records all product ions of a pre-defined precursor. DDA automatically selects the most abundant precursor ions for isolation and fragmentation and monitors all product ions. DIA isolates precursors into pre-defined isolation mass windows and fragments all precursors in each window to generate product ions being monitored by an HRAM mass analyzer.