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Chalcone isomerase activity from (A) purified wild-type recombinant protein and a version containing the bmr30-1 mutation, and (B) plant stalk extracts of wild type, bmr30-1, and bmr30-2. CHI activity levels were assayed using naringenin chalcone substrate. The enzyme velocity was normalized to the amount of protein (mg) added to the reaction. Error bars represent standard error. Samples with different letters are statistically different at α ≤ 0.05 using Tukey’s HSD test.
