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. 2021 Dec 2;12:732307. doi: 10.3389/fpls.2021.732307

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Copyright © 2021 Tetreault, Gries, Liu, Toy, Xin, Vermerris, Ralph, Funnell-Harris and Sattler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stover from mature wild-type (BTx623), bmr30-1, and bmr30-2 plants was subjected to fiber analysis to determine percent of (A) neutral detergent fiber (NDF), (B) acid detergent fiber (ADF), and (C) acid detergent lignin (ADL). The NDF, ADF, and ADL were determined using ANKOM fiber analyzer. Stover was also subjected to (D) bomb calorimetry to determine total energy using a Parr 6400 bomb calorimeter. Values presented are least squares means (±1 SE). Samples with different letters are statistically different from one another at α ≤ 0.05 using Tukey’s HSD test.