Fig. 3.

VDR or ERp57 knockdown by siRNA reduced VDR or ERp57 protein expression, respectively, but did not affect the expression of the non‐knocked‐down protein. Representative Western blots showing VDR (A) or ERp57 and VDR (D) in human primary keratinocytes 48 hours following transfection with siRNA targeted to VDR mRNA (siVDR) (A, lanes 4–6), siRNA targeted to ERp57 (siERp57) (D, lanes 4–6), or a nondirected siRNA sequence (siCTRL) (A, D, lanes 1–3). β‐actin is shown as the loading control. Representative photomicrographs of keratinocytes stained for VDR or ERp57 following transfection with siVDR (B) or siERp57 (E) or siCTRL. Image analysis of IHC images presented as positive VDR (C) or ERp57 (F) protein expression as a percentage of total cellular area 48 hours after transfection with siVDR, siERp57, or siCTRL. Results were from a single experiment performed in triplicate, representative of two separate experiments with similar results. Mean + SEM; ***p < 0.001, when compared to siCTRL vehicle. IHC = immunohistochemistry; NS = not significant between data sets.