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. 2021 Dec 3;5(12):e10581. doi: 10.1002/jbm4.10581

Fig. 3.

Fig. 3

Steps in the liquid chromatography tandem mass spectrometry (LC‐MS/MS) of vitamin D metabolites when using a derivatization technique and an anti‐1,25‐(OH)2D3 antibody to detect low‐abundance forms.