Table 2.

Primer-probe sets after base substitution.

Name	Sequence (5’-3’)	Length (bp)	Amplicon size (bp)
KPC-4-F	AACGCCGCCGCCAATTTGTTGCTGAAGGAG	30	269
KPC-4-mR	5’-Biotin-ATGCGGTGGTTGCCGGTCGTGTTTGCCTTT	30
KPC-mP	5’-FITC-GCGATACTACGTTCCGTCTGGATCGCTGGG [THF]GCTGGAGCTGAACTC-/C3-spacer/-3’	46	196
NDM-1-F	ATGCTGAATAAAAGGAAAACTTGATGGAAT	30	238
NDM-1-mR	5’-Biotin-GCCCCGAAACCCGTCATGTCGAGACAGGAA	30
NDM-mP	5’-FITC-AATAAAAGGAAAACTTGATGGAATTGCCCA A[THF]ATTATGCACCCGATC-/C3-spacer/-3’	47	232
OXA-2	GTAGACAGTTTCTGGCTCGACGGTGGTATT	30	327
OXA-2-R	5’-Biotin-TTCCTGTTTGAGCACTTCTTTTGTGATGGC	30
OXA-mP	5’-FITC-TCGAACCTATGATTGGCTGGTGGGTCGGTT [THF]GGTTGAACTTGATGA-/C3-spacer/-3’	46	140
VIM-4-F	TTCATAGTGACAGCACGGGCGGAATAGAGT	30	258
VIM-4-R	5’-Biotin-CGTACGGTTTAATAAAACAACCACCGAATA	30
VIM-mP	5’-FITC-CAATCCATCCCCACGAATGCGTCTGACTTA [THF]CTAATGAGCTGCTGA-/C3-spacer/-3’	46	217

Modified bases are in red. F represents forward primer, R signifies reverse primer, P means probe and m indicates modified.