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. 2021 Dec 2;12:784975. doi: 10.3389/fimmu.2021.784975

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Copyright © 2021 Xiao, Liang, Liu, Zhang, Xie, Kong, Guo, Wang, Fu, Ye, Li, Zhang, Zhang, Kaudimba, Wang, Kong, Zhao, Zheng and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Organoid cultures derived from patients with Hashimoto’s thyroiditis (HT) and papillary thyroid carcinoma (PTC). (A) Time-lapse imaging sequence of Control, HT, and PTC organoids. Scale bar, 100 μm. (B) Quantitative PCR (qPCR) analysis of the HT and chemokine characterization marker mRNA level in thyroid organoids (Normal, n = 4; HT, n = 4). (C) qPCR analysis of the HT characterization marker mRNA level in thyroid organoids (Normal, n = 3; HT, n = 5; PTC, n = 6). All data are presented as mean ± SEM. (D) H&E staining of the organoids and their corresponding tissues, respectively. Scale bar, 50 μm. Error bars show the mean ± SEM. *P < 0.05; **P < 0.01; ****P < 0.00001.