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. 2021 Dec 1;23(1):99. doi: 10.3892/etm.2021.11022

Figure 1.

Figure 1

Ischemia/reperfusion conditions increases HOTAIR expression. (A) Reverse transcription-quantitative PCR analysis of HOTAIR expression in patients with HIE. ***P<0.001 vs. Healthy. (B) After hBMVECs were subjected to OGD/R, HOTAIR expression was measured using RT-qPCR. **P<0.01 vs. Control and ###P<0.001 vs. OGD/R 12 h. (C) RNA co-immunoprecipitation was performed to evaluate the potential interaction between HOTAIR and EZH2. ***P<0.001 vs. IgG. (D) The effects of siRNA-HOTAIR transfection on HOTAIR expression were measured using RT-qPCR. ***P<0.001 vs. siRNA-NC. (E) The expression of EZH2 was measured by western blotting after siRNA-HOTAIR transfection in hBMVECs treated with OGD/R. ***P<0.001 vs. Control. ###P<0.001 vs. OGD/R 24 h + siRNA-NC. HOTAIR, Hox transcript antisense intergenic RNA; EZH2, enhancer of zeste homolog 2; si, small interfering RNA; OGD/R, oxygen-glucose deprivation/reperfusion; NC, negative control; hBMVECs, human brain microvascular endothelial cells; RT-qPCR, reverse transcription-quantitative PCR.