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. 2021 Dec 1;23(1):97. doi: 10.3892/etm.2021.11020

Figure 2.

Figure 2

PBX1 is a direct target of miR-141-3p in CAL27 cells. (A) Predicted duplex formation of PBX1-wt-3'-UTR and miR-141-3p. (B) Luciferase gene reporter assay results in cells co-transfected with PBX1-wt and miR-141-3p mimic or mimic NC, or co-transfected with PBX1-mt and miR-141-3p mimic or mimic NC. (C) Expression levels of miR-141-3p in cells transfected with miR-141-3p mimic, mimic NC, miR-141-3p inhibitor or inhibitor NC was analyzed by RT-qPCR. (D) PBX1 expression in cells transfected with miR-141-3p mimic, mimic NC, miR-141-3p inhibitor or inhibitor NC was analyzed by western blotting. *P<0.05 vs. mimic NC. #P<0.05 vs. inhibitor NC. wt, wild type; mt, mutant; miRNA/miR, microRNA; NC, negative control; UTR, untranslated region; PXB1, pre-B-cell leukaemia homeobox-1.