Fig. 4. Limited proteolysis of allergen–SiO2 NP conjugates vs. Bet v 1 only. (a) Schematic representation of the experimental setup. (b) Sequence coverage maps of the ten most intense (by relative abundance) proteolytic peptides upon 5 s partial digestion using trypsin and legumain. (c) Sequence coverage maps of the ten least intense (by relative abundance) proteolytic peptides upon 5 s partial digestion using trypsin and legumain. MSNP–allergen conjugates in red, NSNP conjugates in blue, and unbound Bet v 1 in black.
