. 2021 Dec 1;13(48):20508–20520. doi: 10.1039/d1nr05958k

Amount of recombinant protein and NPs used in the different experiments.

Experiment	Concentration MSNPs	Concentration NSNPs	Concentration Bet v 1	Protein/NP ratio
Binding capacity	100 μg mL⁻¹	100 μg mL⁻¹	10–80 μg mL⁻¹	10–80%
CD spectroscopy	1 mg mL⁻¹	1 mg mL⁻¹	100 μg mL⁻¹	10%
Two-step ACE	4 mg mL⁻¹	8 mg mL⁻¹	1 mg mL⁻¹	12.5–25%
Limited proteolysis MS	400 μg mL⁻¹	1 mg mL⁻¹	100 μg mL⁻¹	10–25%
Bet v 1 ELISA	400 μg mL⁻¹	1 mg mL⁻¹	100 μg mL⁻¹	10.25%
huRBL	0.0004–4000 ng mL⁻¹	0.01–10 000 ng mL⁻¹	0.0001–1000 ng mL⁻¹	10–25%