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. 2021 Dec 2;9:788422. doi: 10.3389/fcell.2021.788422

FIGURE 1.

FIGURE 1

The lens of engineered LssG589S/G589S mice could recapitulate the phenotype of human congenital cataract. (A) Schematic diagram of Lss G589S knock-in mice generated at exon 19 of Lss via the CRISPR/Cas9 system. (B) Sanger sequencing performed to validate the G589S missense mutation (GGC > TCC) in WT, LssG589S/+, and LssG589S/G589S mice. Underlined sequences indicate the changed nucleic acids. (C) The amino acid residue G588 in human LSS is highly conserved across species, which is homologous to mouse G589. Blue box refers to the homologous site of LSS G588 in Homo sapiens, Mus musculus, Rattus norvegicus, and Canis lupus. (D) Mouse lens were examined by slit lamp. Red arrows indicated congenital cataract formed in lens of LssG589S/G589S mice at P0. (E) Lenses were isolated and photographed by a dissecting microscope. Red arrows indicated cataract formed in the lens of LssG589S/G589S mice at P0.