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. 2021 Dec 2;9:788422. doi: 10.3389/fcell.2021.788422

FIGURE 2.

FIGURE 2

Disrupted lens structure caused by a homozygous Lss G589S mutation at P0. (A) Representative hematoxylin and eosin staining images of lens structure in WT, LssG589S/+, and LssG589S/G589S mice at P0. Boxes of numbers 1–4, 5-8, and 9–12 indicate four continuous and comparable zones from the lens equator to OFZ within the lens fiber cell regions of WT, LssG589S/+, and LssG589S/G589S, respectively (the leftmost panel, black boxes). Scale bar: 100 μm. The enlargements are the magnifications of the boxed inset shown in the left panel (numbers 1–4, 5–8, and 9–12). Scale bar: 10 μm. Red triangles indicate debris and bulks of fibers in LssG589S/G589S mouse lens. Black arrows indicate that a large number of fiber cell nuclei were still retained at the OFZ region of LssG589S/G589S mouse lens. (B) Electron micrographs of lens morphology at the anterior pole, equator, and OFZ regions in WT, LssG589S/+, and LssG589S/G589S mice. n = 4 lenses per group. At the anterior pole region, representative images of the lens epithelial–fiber interface (EFI) were shown. Red solid lines indicate lens epithelial–fiber interface (EFI) in WT and LssG589S/+ mice. Red dashed line indicates lens EFI in LssG589S/G589S mice. Boxes indicate morphology of lens epithelial cells and EFI in WT, LssG589S/+, and LssG589S/G589S mice (left panel). Scale bar: 10 μm. The enlargement is the magnification of the boxed inset shown in the left panel. Scale bar: 5 μm. At the equator region, representative images of alignment and morphology of secondary fiber cells were shown. Boxes indicate the morphology of secondary fiber cells (left panel). Scale bar: 10 μm. The enlargement is the magnification of the boxed inset shown in the left panel. Scale bar: 5 μm. At the OFZ region, representative images of alignment and morphology of fiber cells were shown. Scale bar: 10 μm. The enlargement is the magnification of the boxed inset shown in the upper panel. Red triangle indicates high-density deposits of fiber debris, and red arrows indicate condensed nuclei in the central OFZ of LssG589S/G589S lens. Scale bar: 5 μm.