Table I.
Changes in expression of genes that affect B cell functions in Runx1 c-k/o measured by RNA-seq analysis of resting B cells and after 3-h activation with anti-IgM + IL-4
Gene | Base Mean | Fold Change 0 h | Fold Change 3 h | Functions | References |
---|---|---|---|---|---|
Ptpn22 | 995 | 1.3 ↓ | 1.9 ↓ | Phosphatase; regulates SRC-family kinase; negative regulator of BCR signaling and B cell apoptosis | Armitage et al. (79) |
Lrrk2 | 6056 | 1.7 ↓ | 3.7 ↓ | Kinase; involved in regulation of B cell homeostasis | Kubo et al. (80) |
Ccr6 | 974 | 3.0 ↓ | 4.0 ↓ | Chemokine receptor; expressed on naive and memory B cells | Krzysiek et al. (81), Bowman et al. (82) |
Cd22 | 11,926 | 1.2 ↓ | 1.4 ↓ | Inhibitory coreceptor; negative regulator of BCR signaling | O’Keefe et al. (74), Müller and Nitschke (83) |
Irf7 | 1046 | 2.0 ↑ | 3.5 ↑ | Regulation of IFN response gene transcription | Ning et al. (84) |
Irf5 | 3126 | 1.1 ↑ | 1.4 ↑ | Regulation of IFN response gene transcription | Ban et al. (85) |
Ifnar1 | 4324 | 2.5 ↑ | 1.9 ↑ | Subunit of IFN-α receptor | Pogue et al. (86) |
Zbp1 | 787 | 1.1 ↑ | 2.1 ↑ | Encodes DNA-dependent activator of IFN-regulatory factors | Takaoka et al. (87) |
Trib1 | 1081 | 1.1 ↑ | 1.9 ↑ | Negative regulator of Ab production | Simoni et al. (88) |
Lgals9 | 1512 | 1.2 ↑ | 1.7 ↑ | Negative regulator of BCR signaling | Cao et al. (66) |
Stat1 | 3506 | 1.0 ↑ | 1.5 ↑ | Enhancement of IFN-induced gene expression | Cheon et al. (89) |
Tnfrsf13c | 4888 | 1.2 ↑ | 1.4 ↑ | Encodes BAFF receptor; promotes survival of FO and MZ B cells | Thompson et al. (90), Schiemann et al. (91) |
Bank1 | 7290 | 1.0 ↑ | 1.3 ↑ | Inhibition of CD40- and BCR-mediated activation of B cells | Aiba et al. (92) |
Adjusted p values for all differences shown < 0.05 (see Supplemental Tables I and II for the full set of base mean values). Arrows indicate fold downregulation (↓) or upregulation (↑) in the Runx1 c-k/o B cells.