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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Alcohol. 2020 Nov 4;91:21–27. doi: 10.1016/j.alcohol.2020.10.005

Figure 1. Determining the effects of chemogenetic manipulation of the NAc core on drinking in C57BL/6J male mice using Drinking in the Dark (DID) paradigm.

Figure 1.

(A) Coronal section 1.34 mm anterior of Bregma (modified from Franklin & Paxinos, 2007) demonstrating target region for stereotaxic delivery of AAV2 hM3Dq, hM4Di, or GFP. Inset shows representative image (4x magnification) of viral expression in the NAc core. (B) Mice underwent a 7-week DID schedule with four distinct DID periods and a 2-week period of no testing (weeks 3-4). Mice received an injection (i.p.) of either vehicle or clozapine-n-oxide (CNO; 1 mg/kg) at zeitgeber time (ZT) 14.5 (30 minutes before DID). From ZT 15-17, mice had access to one of the following fluids: ethanol (20% v/v; weeks 1-2), quinine (0.03 or 0.06 mM; week 5), sucrose (2.5% or 5% w/v; week 6), or water (week 7).