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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: J Allergy Clin Immunol. 2021 Apr 20;148(3):895–901.e6. doi: 10.1016/j.jaci.2021.04.012

Figure 3: Source of IL-17A in peripheral blood after stimulation.

Figure 3:

A. T cell subset activation in peripheral blood as defined by CD69 upregulation. B. To assess potential sources of IL-17, PBMCs from active or outgrown FPIES were stimulated with anti-CD3/CD28 or PMA for 6 h. IL-17+ live singlets were gated, and expression of cell surface markers compared to all live singlets. Left: Quantification of enrichment of each marker across 8 samples. Right: Representative heat map of the transformed ratio of expression of markers under CD3/28 or PMA stimulation conditions comparing IL-17+ cells to all live singlets. C-E: T cell subsets were gated manually and IL-17 (C) and IL-10 (D) expression quantified after stimulation with CD3/CD28 (C,D) or PMA (E). (n=4/group)