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. 2021 Dec 8;6(23):e149699. doi: 10.1172/jci.insight.149699

Figure 6. IL-33–driven immune cell dysfunction is ILC2 dependent.

Figure 6

RAG2–/– mice (ILC2 intact), RAG2–/–αCD90.2 mice (ILC2 depleted), and RAG2–/–IL-2rγ–/– mice (ILC2 deficient) were induced with endometriosis and underwent alternate day i.p. injections of PBS (n = 6) or IL-33 (n = 6). (A) Peritoneal lavage cells harvested from PBS- and IL-33–treated EMS mice were counted using a Countess II FL Automated Cell Counter. (B) Frequency of ST2+ cells in the PBS- and IL-33–treated mice analyzed via flow cytometry. (C) Frequency of ILC2 (singlet, live, CD45+Lineage–CD25+Thy2+, ST2+). (D) Frequency of eosinophils (singlet, live, CD45+CD11b+F4/80+, Siglec-F+). (E) Frequency of LPM (singlet, live, CD45+ CD11b+ Siglec-F– F4/80hi, MHC-IIlo). (F) Frequency of SPM (singlet, live, CD45+CD11b+ Siglec-F–F4/80lo, MHC-IIhi). *P < 0.05, **P < 0.01, ***P < 0.001. Mean ± SD. One-way ANOVA was used.