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. 2021 Dec 8;6(23):e146757. doi: 10.1172/jci.insight.146757

Figure 7. The relationship between miR-1262 and HIF-1α in RA FLSs.

Figure 7

RA FLSs were treated with miRNA inhibitors or mimics or transfected with HIF-1α siRNA (si-HIF-1α-1 or si-HIF-1α-3). The miRNAs and mRNA were detected using RT-qPCR. The protein levels were measured by Western blot. (A) Effect of HIF-1α knockdown on the expression of miR-1262. (B and C) Effect of miR-1262 inhibitor (B) or mimics (C) on mRNA expression of HIF-1α. (D) Effect of miR-1262 inhibitor or mimics on protein expression of total HIF-1α. (E) Effect of miR-1262 inhibitor or mimics on protein expression of nuclear HIF-1α. Data (A–C) are presented as the mean ± SD from at least 3 independent experiments. Graphs (D and E, right panel) are expressed as the mean ± SD of densitometry quantification of Western blot results from at least 3 independent experiments. (F) Proposed model for LINK-A–mediated regulation of the synovial migration, invasion, and inflammation in RA FLSs.