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. 2021 Dec 8;6(23):e150059. doi: 10.1172/jci.insight.150059

Figure 4. GATA4 regulates the expression of fibrogenic and antifibrogenic genes in HSCs.

Figure 4

Immunofluorescence analyses of laminin and GATA4 accumulation in LX2 cells transfected with (A) GFP-expressing (Ad-GFP) (multiplicity of infection [MOI], = 100) and GATA4-expressing (Ad-GATA4) adenovirus at (B) MOI = 50 and (C) MOI = 100. (D) Heatmap showing the most differentially expressed genes in Ad-GATA4–transfected LX2 cells compared with LX2 cells transfected with Ad-GFP. Validation by quantitative RT-PCR analysis of differentially (E) fibrogenic (n = 3–6) and (F) antifibrogenic (n = 3–6) expressed genes (Ad-GATA4 vs. Ad-GFP infected LX2 cells) identified in the microarray analyses. (G) Validation by Western blot analysis of selected differentially expressed genes (Ad-GATA4 vs. Ad-GFP infected LX2 cells) identified in the microarray analyses. β-Actin and GAPDH proteins were used as loading controls. Quantitative RT-PCR analysis of (H) fibrogenic (n = 4–8) and (I) antifibrogenic genes (n = 3–6) in G2-Cre;Gata4 KO E13.5 embryonic liver. Scale bars: 25 μm. Statistical analyses was performed using 2-tailed Student’s test. Error bars represent mean ± SEM. *P < 0.05, **P < 0.01.