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. 2021 Dec 8;6(23):e146334. doi: 10.1172/jci.insight.146334

Figure 2. Mechanism of NSCLC cell radioresistance induced by SL-CAFs.

Figure 2

(A) Volcano plot showing the upregulated, downregulated, or insignificantly differential expressed genes of H292 cells cultured with CAF CM or SL-CAF CM for 48 hours. (B) Heatmap illustrating changes of tumor growth– and progression-related gene sets. (C) KEGG pathway enrichment revealing the activation of JAK/STAT and cytokine-cytokine receptor interaction signaling pathways as indicated in H292 cells cultured with SL-CAFs. (D) Representative Western blot for protein expression levels of pSTAT3, STAT3, pJAK2, JAK2, and MYC in NSCLC cells. (E) STAT3 knockdown in NSCLC cells with siRNA and detection of downstream pathway MYC molecules by Western blot. (F) NSCLC cell apoptosis rate 72 hours after 8 Gy IR detected by flow cytometry as indicated in different groups. The indicated results represent the mean ± SEM of 3 independent experiments, analyzed by 1-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001. SL-CAFs, senescence-like CAFs.