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. 2021 Oct 28;6(23):e147371. doi: 10.1172/jci.insight.147371

Figure 8. Persistent Na+ current induces RyR2 dysfunction, which is attenuated by expression of mitochondrial catalase.

Figure 8

(A) Representative anti-RyR, anti-DNP, anti-Calstabin2, and anti–phospho-Ser2808 (p2808) and Ser2814 (p2814) antibody immunoblots of RyR2 immunoprecipitates from atrial tissue of nontransgenic, F1759A, and F1759A-mCAT mice. See complete unedited blots in the supplemental material. DNP, 2,4-dinitrophenol. (B) Graphs of quantification normalized to immunoprecipitated RyR2. P < 0.0001 by 1-way ANOVA for DNP, Calstabin2, and p2808, P < 0.001 for p2814. **** P < 0.0001, *** P < 0.001, ** P < 0.01 by Tukey’s multiple-comparison test. (C and D) Representative Ca2+ spark images and graph of Ca2+ spark frequencies from isolated cardiomyocytes from atria of nontransgenic controls, F1759A, and F1759A-mCAT mice. From left to right, n = 58, 41, and 56 atrial cardiomyocytes from 3 mice in each group. P < 0.0001 by 1-way ANOVA; **** P < 0.0001 by Tukey’s multiple-comparison test.