Figure 2. Catalytic reduction of ketones with hCAII in whole cells.

a, Catalytic activity of purified protein (green), wet whole cells (cyan), stored wet whole cell (yellow) and lyophilized whole cell (brown). b, Enantioselective reduction of 4-acetylpyridine and acetophenone by deuterated phenyl silane. c, The yield and enantioselectivity of reaction product from whole-cell reduction of 4-acetylpyridine on gram scale. The picture is a snapshot of the gram-scale reaction.