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HIRA is a nuclear protein. Asynchronously growing U2OS cells grown on coverslips were stained with a control immunglobulin G1 (IgG1) monoclonal antibody (a) or two anti-HIRA monoclonal antibodies together, WC19 and WC119 (both IgG1) (e). DNA was visualized with DAPI (b and f). U2OS cells grown on coverslips were transfected with a plasmid encoding HA-HIRA[1–1017] and then stained with anti-HA antibody 12CA5 (d and h). DNA was visualized with DAPI (c and g).
