TABLE 1.
Main validation parameters of the HPLC-DAD method applied to quantify escitalopram and paroxetine after in vitro permeation studies.
| Drug | Mobile phase | Flow rate (ml/min) | Run time (min) | Injection volume | Detection wavelength (nm) | Retention time (min) | Calibration range (µg/ml) | Coefficient of determination (r 2) | LLOQ (µg/ml) | Precision (% CV) a | Accuracy (% Bias) a |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Escitalopram | 20.0 mM phosphate buffer pH 3.80 with orthophosphoric acid/ACN (70.0:30.0, v/v) | 1.00 | 8.00 | 20.0 | 240 | 2.89 | 0.100–1.00 | 0.997 | 0.100 | 5.01–11.4 | −6.62–−4.48 |
| Paroxetine | 290 | 3.56 | 0.100–2.00 | 0.996 | 0.100 | 9.68–13.7 | −11.0–12.2 |
ACN, acetonitrile; Bias, deviation from nominal value; CV, coefficient of variation; LLOQ, lower limit of quantification.
a
Inter-day values (n = 3).