TABLE 4.
Main validation parameters of the high-performance liquid chromatography (HPLC) method applied to quantify paroxetine in plasma, brain, and lung matrices.
| Validation parameter | Mouse matrices (n = 3) | ||
|---|---|---|---|
| Plasma | Brain homogenate | Lung homogenate | |
| Calibration range (µg/ml) | 0.0500–2.00 | 0.0250–1.00 | 0.0500–2.00 |
| Regression equation a | Y = 1.19–0.00671 | Y = 1.96 + 0.00428 | Y = 1.14–0.0158 |
| Coefficient of determination (r 2) b | 0.994 | 0.996 | 0.994 |
| LLOQ (µg/ml) | 0.0500 | 0.0250 | 0.0500 |
| Intra-day | |||
| Precision (%CV) | 7.07–14.3 | 1.25–7.98 | 10.0–13.4 |
| Accuracy (%RE) | −10.9–0.865 | −3.11–13.2 | −9.84–9.72 |
| Intra-day | |||
| Precision (%CV) | 6.90–12.2 | 4.97–9.94 | 5.73–14.6 |
| Accuracy (%RE) | 0.974–14.9 | −3.31–12.5 | −14.7 to −1.71 |
LLOQ, lower limit of quantification; CV, coefficient of variation; %RE, deviation from nominal value.
a
Equation of the calibration curve is given by the general equation of y = mx + b, with m corresponding to the slope and b to the intercept. The equation represents the peak areas signals of paroxetine to that of the internal standard (y), versus the corresponding concentration of paroxetine (x).
b
Weighted linear regression using 1/×2 as the best weighting factor.