Table 1.
Details of quantitative trait loci (QTLs) identified for pre-harvest sprouting (PHS) resistance
| QTL Name | Chr | Position | Intervalmax | LODmax | Additive effectmax | %R2max | Environment(s) CIM and MCIM | Closest marker and position | Donor allele | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Name | cM | nt | |||||||||
| QPhs.lrdc-1A.1 | 1A | 27.80 | 27.6–30.8 | 2.55 | 0.32 | 5.0 | Ithaca 2018, Pooled | tplb0036l23_696 | 27.59 | 16,056,699 | T |
| QPhs.lrdc-1A.2 | 1A | 60.40 | 58.4–64.0 | 6.66 | 0.63 | 14.0 | Edmonton 2019 | Ku_c44068_601 | 60.27 | 38,215,835 | T |
| QPhs.lrdc-1A.3 | 1A | 81.90 | 79.1–82.7 | 5.14 | 0.62 | 9.0 | Lethbridge 2019, Pooled | Kukri_c25215_124 | 81.85 | 493,658,123 | T |
| QPhs.lrdc-2A | 2A | 106.40 | 105.4–107.4 | 2.85 | 0.63 | 5.0 | Lethbridge 2019 | BS00090569_51 | 106.37 | 657,329,310 | T |
| QPhs.lrdc-2B.1 | 2B | 82.00 | 81.3–82.5a | 5.01 | 1.43 | 10.0 | Edmonton 2019, Pooled | Tdurum_contig1653_190 | 82.00 | 107,712,408 | T |
| QPhs.lrdc-2B.2 | 2B | 90.00 | 86.7–91.6a | NA | 0.84 | 2.1 | Edmonton 2019, Ithaca 2018, Lethbridge 2018, Lethbridge 2019, Pooled | Ku_c69635_2786 | 89.96 | 110,037,917 | I |
| QPhs.lrdc-2D.1 | 2D | 42.40 | 38.7–45.3 | 2.55 | 0.62 | 5.0 | Ithaca 2018, Lethbridge 2019, Pooled | wsnp_CAP12_c1503_764765 | 42.37 | 37,927,516 | T |
| QPhs.lrdc-2D.2 | 2D | 101.30 | 98.8–102.2 | 3.40 | 0.34 | 6.0 | Ithaca 2018 | Ex_c2115_3369 | 104.08 | 437,187,123 | T |
| QPhs.lrdc-3A.1 | 3A | 8.20 | 6.3–9.3 | 12.00 | 1.16 | 19.0 | Edmonton 2019, Ithaca 2018, Lethbridge 2018, Pooled | Tdurum_contig83209_316 | 7.61 | 1,253,899 | T |
| QPhs.lrdc-3A.2 | 3A | 19.60 | 18.6–27.9 | 4.82 | 0.84 | 9.0 | Lethbridge 2019 | Ra_c9347_163 | 19.54 | 35,795,572 | T |
| QPhs.lrdc-3B.1 | 3B | 1.70 | 0.7–2.3 | 2.89 | 0.53 | 4.0 | Lethbridge 2018, Pooled | BS00001400_51 | 1.69 | 860,129 | I |
| QPhs.lrdc-3B.2 | 3B | 157.30 | 156.1–162.8 | 7.20 | 0.59 | 13.0 | Ithaca 2018, Lethbridge 2019, Pooled | BS00057988_51 | 157.58 | 774,477,527 | T |
| QPhs.lrdc-3D.1 | 3D | 17.20 | 12.4–32.9 | 4.21 | 0.80 | 10.0 | Lethbridge 2018 | wsnp_Ex_c7780_13254349 | 18.67 | 346,008,587 | I |
| QPhs.lrdc-3D.2 | 3D | 122.20 | 107.6–138.4 | 6.18 | 0.48 | 12.0 | Ithaca 2018, Pooled | BS00067163_51 | 117.07 | 573,581,940 | T |
| QPhs.lrdc-4A | 4A | 45.60 | 45.3–48.9 | 6.14 | 0.78 | 9.0 | Edmonton 2019, Ithaca 2018, Lethbridge 2018, Pooled | wsnp_Ex_rep_c67799_66488792 | 45.53 | 65,464,862 | T |
| QPhs.lrdc-4B | 4B | 61.00 | 60.6–63.1 | 3.83 | 0.61 | 6.0 | Lethbridge 2018, Pooled | wsnp_Ex_c25373_34639805 | 60.99 | 481,850,259 | T |
| QPhs.lrdc-4D | 4D | 74.00 | 72.1–75.6 | 2.60 | 0.52 | 4.0 | Lethbridge 2018 | BS00047797_51 | 76.15 | 456,267,708 | I |
| QPhs.lrdc-5A.1 | 5A | 57.10 | 56.4–57.3 | 2.61 | 0.36 | 4.0 | Pooled | GENE-3502_206 | 57.04 | 160,103,231 | I |
| QPhs.lrdc-5A.2 | 5A | 123.60 | 123.6–123.6 | 2.50 | 0.37 | 5.0 | Edmonton 2019 | BS00065292_51 | 123.50 | 556,978,174 | T |
| QPhs.lrdc-7A | 7A | 192.20 | 190.8–193.9 | 2.65 | 0.49 | 4.0 | Ithaca 2018, Lethbridge 2018, Lethbridge 2019, Pooled | TA003093–1305 | 192.01 | 637,205,996 | T |
| QPhs.lrdc-7D | 7D | 89.20 | 70.5–106.6 | 6.21 | 1.20 | 18.0 | Lethbridge 2019, Pooled | D_GCE8AKX02ILA1U_88 | 79.82 | 56,638,676 | I |
Note - Chr chromosome, Intervalmax QTL interval (cM) calculated using markers identified in composite interval mapping (CIM) or mixed-model based composite interval mapping (MCIM) based on all the environments; ‘cM’ and ‘nt’ positions are based on AAC Innova/AAC Tenacious linkage map and IWGSC RefSeq v.2 physical map/genome, respectively
aQTL interval based on MCIM results only; LODmax, Additive effectmax and %R2max: highest score reported in any single environment or pooled data, additive effect is shown as absolute value; NA: QTL detected using MCIM only and no LOD score was calculated; Donor allele: T – AAC Tenacious, I – AAC Innova