Skip to main content
. 2021 Dec 16;17(12):e1009938. doi: 10.1371/journal.pgen.1009938

Fig 3. Knock-down of ChT in MB intrinsic neurons impairs spontaneous recovery and dishabituation of habituated larvae.

Fig 3

Scatter plot shows response indices towards ETA of larvae pre-exposed to 5 min ETA (Post-hab). For spontaneous recovery, the larvae were pre-exposed to 5’ETA and R.I was calculated after 15, 30 or 60 min rest time in absence of stimulus. For dishabituation, the larvae were pre-exposed to 5’ETA and R.I was calculated after 1 min cold shock (5’ETA+coldshock). Scatter plot also shows R.I of naïve larvae presented to 1 min cold shock (naïve+ cold shock). Scatter plot shows R.I in the described conditions in genotypes: (A) NP1131> UAS-ChTRNAi1 (pink circles), NP1131> UAS-ChTRNAi1;UAS-ChT (blue circles) as compared to their controls NP1131> + (black circles). NP1131> UAS-ChTRNAi2 (pink circles) as compared to UAS-ChTRNAi2>+ (black circles). (B) C305a> UAS-ChTRNAi1 (pink circles), C305a> UAS-ChTRNAi1;UAS-ChT (blue circles) as compared to their controls C305a> +(black circles). C305a> ChTRNAi2 (pink circles) as compared to UAS-ChTRNAi2>+ (black circles). (C) MB247> UAS-ChTRNAi1 (pink circles), MB247> UAS-ChTRNAi1;UAS-ChT (blue circles) as compared to their controls MB247> + (black circles). MB247> UAS-ChTRNAi2 (pink circles) as compared to UAS-ChTRNAi2>+ (black circles). Both spontaneous recovery and dishabituation was impaired in ChT knocked-down group of larvae (data represented by pink circles). Each N represent one experiment performed with a group of 30 larvae. Each data group was analysed for normal distribution using Shapiro-Wilk test. Statistical significance was determined by two-tailed unpaired t-test (parametric) with Welchs correction. *** represent p≤0.0001, ** represent p≤0.001, n.s means statistical non-significance when p≥0.05. For more statistical details and numerical data values in the scatter plot refer to S1 and S2 Data.