Table 3. FAdV isolation and nucleic acid detection in samples obtained from cloacal swabs and/or organs of infected chickens.
FAdV-11 | dpi | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
3 | 4b | 6 | 9 | 13 | 16 | 20 | 23 | 28 | ||
Virus isolation from livers | 5/5a | 1/1 | 5/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/4 | |
DNA detection | Cloacal swabs | 5/5 | NE | 5/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
Liver | 5/5 | 1/1 | 5/5 | 1/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | |
Kidney | 3/5 | 1/1 | 3/5 | 1/5 | 1/5 | 1/5 | 0/5 | 0/5 | 0/5 | |
Spleen | 3/5 | 1/1 | 3/5 | 1/5 | 1/5 | 0/5 | 0/5 | 0/5 | 0/5 |
Samples from orally infected chickens with FAdV-11 were investigated at 3, 6, 9, 13, 16, 20, 23 and 28 days post infection (dpi) by conventional PCR.
aNo. of positive samples / no. of tested samples.
bOrgan samples taken from a dead chicken at 4 dpi.
NE not examined.