Fig 2. Expression of SARS-CoV-2 proteins from rVSV_Ind-SARS-CoV-2.

To check the expression of SARS-CoV-2 RBD, S1, S_F, and E proteins from the rVSV_Ind-SARS-CoV-2 infected cells, BHK-21 cells were infected with the virus at an MOI of 6. After six hours incubation at 37°C, cell lysates were prepared and protein expression was determined by Western blot analysis. Cell lysates were loaded in 5 μg quantity for SDS-PAGE. RBD, S1, and S_F proteins were detected by rabbit antibody against SARS-CoV-2 RBD. S2 protein was detected by rabbit antibody against SARS-CoV-2 S2. E protein was detected by rabbit antibody against SARS-CoV-2 E peptides. (A) Expression of RBD, S1, and S_F with and without msp and Gtc. (B) Expression of S2 with and without Gtc. (C) Expression of E protein. (D) Expression of VSV_Ind N, P, M, and G proteins. Purple box: honeybee msp, red box: VSV Gtc.