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. 2021 Dec 16;16(12):e0261127. doi: 10.1371/journal.pone.0261127

Fig 4. Met restrains F-actin rings formation and bone resorption of osteoclast in vitro.

Fig 4

(A) BMMs were stimulated with 30 ng/ml M-CSF, 50 ng/ml RANKL, and various concentrations of Met for 7 d and then stained with TRITC-conjugated phalloidin and DAPI to show F-actin rings and nucleus. Scale bar, 50 μm. (B) BMMs were seeded on bone slices and stimulated with 30 ng/ml M-CSF, 50 ng/ml RANKL, and various concentrations of Met for 9 d, bone resorption areas stained with toluidine blue were examined. Scale bar, 50 μm. (C) Bone resorption pits were shown by SEM. Scale bar, 20 μm. Quantitative analysis of (D) F-actin rings, (E) resorption areas and (F) resorption pits. n = 3 per group. **P < 0.01 and ***P < 0.001, compared with control group.