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. 2021 Dec 2;15:786329. doi: 10.3389/fnana.2021.786329

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Copyright © 2021 Fang, Wang and Naumann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overview of technical approach. (A) Schematic diagram of EdU injections in pregnant rats from E12.5 to E17.5, followed at P0 by tissue collection, microtomy, and staining. (B) Relation of P0 rats survival rate with EdU concentrations and (left graph), and with survival time after EdU injection (right graph). EdU labeled P0 dorsal (C) and lateral (D) cortical sections from E12.5 to E17.5 injections. (E) Nurr1 expression in P0 sections from anterior to posterior, and magnified Nurr1 labeled regions including, dCL (dorsal claustrum), vCL (ventral claustrum), dDEn (dorsal DEn), vDEn (ventral DEn), dLn (deep layer neurons), sLn (superficial layer neurons), and pDEn (posterior DEn). Scale bar in panels (C,D) = 100 μm. Scale bar in panel (E) = 0.5 mm.