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. 2021 Dec 2;15:773375. doi: 10.3389/fncel.2021.773375

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Copyright © 2021 Wang, Gu, Gan, Tian, Zhang, Zhao and Lei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Survival and differentiation of NGF-modified NSCs after transplantation and high levels of NGF at the lesion core. (A) Four weeks after NGF-NSCs transplantation, we observed that NGF + /ZsGreen + cells aggregated in the epicenter of the injured spinal cord, and several of them migrated into the peri-lesion area. In comparison, the sham and control rats did not show a positive signal of ZsGreen, and their immunoactivity of NGF was much weaker. (B) The transplanted NGF-NSCs marked by ZsGreen expressed β-tubulins3 and CNPase, which indicated the NGF-NSCs could differentiate into neurons and oligodendrocytes. (C,D) The western blotting analysis demonstrated that NGF’s protein level was much higher in the NGF-NSCs groups at day 14, 21, 28, and 35 after injury. (E) Similarly, the mRNA level of NGF exhibited the same elevation in the three groups. Scale bar, white bar 5 μm; yellow bar 200 μm. ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001.