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. 2021 Dec 3;5(3):e202101183. doi: 10.26508/lsa.202101183

Figure 1. β-arrestin1 is required for mGluR2- and β2AR-mediated increase in pathogenic tau.

Figure 1.

(A) HeLa-V5-tau cells were transfected with control siRNA or β-arrestin1 siRNA. After transfection, the cells were treated with either vehicle or 10 μM isoproterenol (ISO), lysed, and immunoblotted for indicated proteins. Representative blots are shown. (B) Quantification of phospho-tau, pS396/pS404-tau (PHF1) levels. n = 3 independent experiments. *P < 0.05. One-way ANOVA with Dunnett’s test. (C) HeLa-V5-tau cells were transfected with control siRNA or β-arrestin1 siRNA and treated with either vehicle, 1 or 10 μM LY-379,268, lysed, and immunoblotted for indicated proteins. Representative blots are shown. (D) Quantification of phospho-tau (PHF1) levels. n = 4 independent experiments. **0 < 0.005. One-way ANOVA with Dunnett’s test. (E) HeLa-V5-tau cells were transfected with control siRNA or β-arrestin2 siRNA and treated with either vehicle, 10 μM isoproterenol (ISO), or 10 μM LY-379,268 (LY), lysed, and immunoblotted for indicated proteins. (F) Quantification of phospho-tau (PHF1) levels. n = 4 independent experiments. *P < 0.05, **P < 0.005. One-way ANOVA with Dunnett’s test.