Figure S4. Reduced β-arrestin1 mitigates depletion of synaptic protein in PS19 primary neurons.

(A) Hippocampal primary neurons derived from WT and PS19 P0 pups were transduced with either GFP or β-arrestin1-shRNA-GFP lentivirus on DIV5. Neurons were stained with synaptophysin on DIV21 (Scale bar = 10 μm). Representative images are shown. (B) Quantification of synaptophysin intensity. n = 4 independent experiments, #P < 0.0001. One-way ANOVA with Dunnett’s test.