Figure S6. Increased β-arrestin1 impairs LC3-mediated autophagy.

(A) HeLa-V5-tau cells were transfected with control vector or β-arrestin1-myc and treated with vehicle or 100 nM of Bafilomycin A1 for 4 h. Cells were fixed and immunostained for myc and LC3 (Scale bar = 10 μm). Representative images are shown. (B) Quantification of LC3 puncta area normalized to vector control vehicle condition. n = 4 independent experiments. #P < 0.0001. One-way ANOVA with Dunnett’s post hoc test. All data are presented as mean ± SEM. (C) HeLa-V5-tau cells were transfected with GFP or GFP-β-arrestin1 and/or HA-p62, subjected to co-IP for HA, and immunoblotting for GFP, HA, and actin.